“BioProzessTrainer” as training tool for design of experiments

Concept Design and optimization of cell culture processes requires intensive studies based on “Design of experiments”-strategies. In academia teaching of DoE-concepts is often insufficient, as in most cases only simple culture strategies (batch) can be performed, as time and money are limited. More complex tasks such as feeding strategies for fed batch culture can be discussed theoretically only. To close this gap the virtual “BioProzessTrainer”, a model based simulation tool, was developed. It supports biotechnological education with respect to process strategies, bioreactor control, kinetic analysis of experimental data and modeling. Along with a set of examples for different control and process strategies (batch, fed batch, chemostat etc.) learners are prepared for real experiments [1,2]. The “BioProzessTrainer” (Figure 1) helps to improve the quality of education by using interactive learning forms and by transmitting additional knowledge and skills. Costs for practical experiments can be minimized by reducing plant operation costs. Here a concept for teaching DoE-concepts for batch(optimization of e.g. substrate concentrations and inoculation cell density) and fed-batch-processes (evaluation and optimization of feeding strategy) using the “BioProzessTrainer” is shown.